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Web18 mar 2024 · Introduction. Alternative splicing (AS) generates multiple functional mRNAs and therefore enables different proteins to be synthesized from one single gene (Maniatis and Tasic, 2002; Nilsen and Graveley, 2010).This regulatory mechanism significantly expands transcript variability and proteome diversity, as well as provides additional … Web23 mar 2009 · The kidney injury molecule-1 (designated as Kim-1 in rodents, KIM-1 in humans) mRNA was identified using techniques of representational difference analysis, a PCR-based technique [ 1 ], which we carried out to find genes whose expression was markedly upregulated 24–48 h after ischaemia in the rat [ 2 ]. Web1 mag 2012 · Abstract. The genes period (per) and timeless (tim) are core components of the circadian clock that regulates a wide range of rhythmic biochemical, physiological, and behavioral processes in prokaryotes and eukaryotes.We used degenerate polymerase chain reaction (PCR) and Rapid Amplification of cDNA Ends (RACE) to clone and sequence … chloral hydrate discovery