Phospholipidosis detection reagent

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August undefined, 2024

WebJan 1, 2014 · Phospholipidosis (PLD) is characterized by excessive intracellular accumulation of phospholipids, with a distinct morphological appearance of lamellar bodies visible under an electron microscope (EM). 1 Drugs, including many antibiotics, antidepressants, and antihistamines, induce PLD in various tissues such as lung, 2 heart, … WebSeveral methods for the detection of phospholipids have been reported (29-37). They are summarized in Table 1. In-vitro screening assays using the fluorescently tagged lipid NBD-PE, the fluorescent probe Nile red as well as the phosphatidylcholine-conjugated dye NBD-PC were previously used to determine intracellular phospholipids (29, 30, 34).

Drug-Induced Phospholipidosis: Best Practices and Screening

WebNov 9, 2024 · Phospholipidosis (PLD), the intracellular accumulation of phospholipids, is an adaptive response to toxic stimuli and serves as an important parameter in the biological … WebThe HCS LipidTOX Red phospholipidosis detection reagent was developed to characterize the potentially toxic side effects of compounds on lipid metabolism in mammalian cell … read aloud books free online

IJMS Free Full-Text Chloroquine-Induced Accumulation of

WebThermo Fisherhcs lipidtox green phospholipidosis detection reagent Hcs Lipidtox Green Phospholipidosis Detection Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Webreagents containing DMSO using equipment and practices appropriate for the hazards posed by such materials. Dispose of the reagents in compliance with all pertaining local … WebThe EM method, the gold standard of detecting cellular phospholipidosis, has downsides, namely, low-throughput, high-costs, and unsuitability for screening a large chemical … read aloud books for grade 1

Different Sensitivity of Macrophages to Phospholipidosis …

Detection of Phospholipidosis Induction: A Cell-Based Assay in …

Webdrug-induced phospholipidosis. The morphological hallmark of phospholipidosis is the appearance of unicentric or multicentric-lamellar bodies when viewed under an electron … WebJul 16, 2015 · For phospholipid staining, the cells were treated with 400 μM palmitate containing 1X LipidTOX Red phospholipidosis detection reagent. For Immunostaining with an anti-calnexin antibody, the cells were fixed in 4 % paraformaldehyde in PBS. The fixed cells were rinsed with PBS and incubated in blocking solution (5 % goat serum, and 0.001 … how to stop hydro hypostasis from healingWebMar 1, 2024 · LipidTOX Green Phospholipidosis Detection Reagent (LipidTOX, Invitrogen, Carlsbad, CA, USA) was applied to confirm induction of phospholipidosis by selected … read aloud books for kids awnie

"WebFeb 27, 2024 · The LipidTOX Green Phospholipidosis Detection Reagent is dedicated to phospholipidosis detection, which is often triggered by cationic amphiphilic drugs. Within 24 h of incubation with CQ, the increase in fluorescence intensity of LipidTOX for phospholipidosis detection was significant. " - Phospholipidosis detection reagent

Phospholipidosis detection reagent

HCS LipidTOX™ Red Neutral Lipid Stain, for cellular imaging

WebInvitrogen’s HCS LipidTOX™ Phospholipidosis and Steatosis Detection Kits offer the reagents for performing image-based high-content screening (HCS) assays to … WebJun 22, 2024 · Third, phospholipidosis is a confound that only affects drugs repurposed for direct antiviral activity—it is irrelevant for drugs like dexamethasone and fluvoxamine that …

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WebPhospholipidosis is an excessive intracellular accumulation of phospholipids and drug, which is normally reversible after discontinuation of drug treatment. Currently, it is … WebThis probe is compatible with the HCS LipidTOX phospholipidosis detection reagents. HCS LipidTOX neutral lipid stains can also be used to monitor the formation and differentiation of adipocytes, a process called adipogenesis. Cell Analysis, Cellular Imaging, High-Content Screening (HCS) Order Info Shipping Condition: Room temperature Specifications

WebOct 30, 2024 · In this chapter, we describe fluorescent reagents and the protocols for the measurement of various parameters such as mitochondrial membrane potential, plasma …

WebThe HCS LipidTOX Red phospholipidosis detection reagent was developed to characterize the potentially toxic side effects of compounds on lipid metabolism in mammalian cell lines. This reagent is easily detected by fluorescence microscopy or an HCS reader equipped with filters that can detect excitation/emission maxima approx. 595/615nm ... WebHCS LipidTOX™ Phospholipidosis Detection Reagents 3 Preparing the Labeling Solution and Staining the Cells 1.1 Dilute the 1000X LipidTOX™ phospholipidosis detection …

WebCell Based Assays Bioassays, kits, and reagents used to analyze various properties of living cell samples. Products include apoptosis assays, signal transduction assays, and cell growth and differentiation assays. General Cell Based Assays (1,186) Signal Transduction Reagents and Kits (1,016) Cell Growth and Differentiation Reagents and Kits (423)

WebApr 1, 2015 · Phospholipidosis (PLD) is a disorder of excessive storage of phospholipids (PLs) in the lysosomes of cells and tissues. Several mechanisms have been described which may lead to the accumulation of PLs in lysosomes and can be due to genetic disorders (e.g. Niemann-Pick disease) or triggered by xenobiotics or drugs, referred to as drug-induced … read aloud books for kids free onlineWebPhospholipidosis (PL) is lysosomal storage disorder characterized by the excessive accumulation of intracellular phospholipids (Figure 1). The mechanism of … read aloud books why are you lyingWebThe HCS LipidTOX™ Red phospholipidosis detection reagent was developed to characterize the potentially toxic side effects of compounds on lipid metabolism in mammalian cell lines. This reagent is easily detected by fluorescence microscopy or an HCS reader … The HCS LipidTOX™ Red phospholipidosis detection reagent was developed to … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes read aloud bubble gum brainWebJan 1, 2014 · Phospholipidosis (PLD) is characterized by excessive intracellular accumulation of phospholipids, with a distinct morphological appearance of lamellar … how to stop hydrolysis in shoesWebLipidTox Green neutral lipid stain and LipidTox Red phospholipidosis detection reagent (Kit from Molecular Probes) ... ORIGINAL ARTICLE 232 E-LDL and Ox-LDL Differ in the Lipid Storage. phospholipidosis detection depending on the emission spectra of the dye (Table 1) (30,37–39). Lipid-loaded macrophage foam cells play a major role in how to stop hydraulic leakWebSep 3, 2012 · Drug-induced phospholipidosis (PLD) is a lysosomal storage disorder characterized by the accumulation of phospholipids within the lysosome. ... After 48 h, the medium was replaced with fresh medium that included the test substances and HCS LipidTOX Green phospholipidosis detection reagent (Invitrogen, San Diego, US) at their … how to stop hydraulic oil leaksWebMar 17, 2024 · This chapter describes a cell-based high-content phospholipidosis assay using the LipidTOX reagent in a high-throughput screening (HTS) platform. This assay … how to stop hydraulic cylinder from leaking