Human monocytic cell line

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August undefined, 2024

WebAbstract Mono Mac 6 is a human monocytic cell line with several features of mature blood monocytes such as CD 14 antigen expression, phagocytotic ability, and the functional … WebCell Line Description Derived from the peripheral blood of a 1 year old male with acute monocytic leukaemia. THP-1 cells have Fc and C3b receptors and lack surface and …

THP-1 Cell-based Assays Monocyte Reporter Cell Lines - InvivoGen

Web24 mei 2024 · Reagents and cell culture. The human monocytic cell line THP-1 [], the human myelogenous leukaemia cell line K562 [] and HEK293 hTLR4A-MD2-CD14 (Invivogen, San Diego, CA) were maintained in RPMI 1640 supplemented with 10% heat-inactivated fetal calf serum (FCS), 100 μg/mL streptomycin and 100 U/mL penicillin.Cells … harry patterson allstate

Toxins Free Full-Text Shiga Toxins: Intracellular Trafficking to ...

WebThe HL-60 cell line is a human leukemia cell line that has been used for laboratory research on blood cell formation and physiology. HL-60 proliferates continuously in … WebArticle Snippet: Human acute monocytic leukemia cell line THP-1 (ATCC TIB-202) was cultured in RPMI 1640 medium (HyClone, Logan, UT, USA) with 10% fetal bovine serum (FBS; Gibco, Carlsbad, CA, USA) at 37 °C in 5% CO 2 . Techniques: Cotransfection, Transfection, Expressing, Quantitative RT-PCR, Western Blot, Infection. WebGenerating primary human monocyte-derived macrophages and dendritic cells may be a suitable alternative to monocytic cell lines in applications where phenotypical relevance is of critical concern, ... Liu M, Wu M. Induction of human monocyte cell line U937 differentiation and CSF-1 production by phorbol ester. Exp Hema 20(8):974-979, 1992. 6. charlene abalos

Human Acute Monocytic Leukemia Cell Line Thp ATCC Bioz

U-937 - CRL-1593.2 ATCC

WebKeywords. Blast Crisis. Mononuclear Phagocyte System. Monocytic Cell Line. Naphthyl Acetate. Diffuse Histiocytic Lymphoma. These keywords were added by machine and not … Web10 mei 2024 · Monocyte like cell lines (MCLCs), including THP-1 and HL-60 cells (derived from patients with acute monocytic leukemia) and U-937 cells (immortalised from a … charlene abendrothWebCell line: MONO-MAC-6 : DSMZ no.: ACC 124: Species validation: human (Homo sapiens)Cell type: acute monocytic leukemia : Origin: established from the peripheral blood of a 64-year-old man with relapsed acute monocytic leukemia (AML FAB M5) in 1985 following myeloid metaplasia; carries t(9;11)(p22;q23) leading to KMT2A-MLLT3 (MLL … c harlene

"Web8 aug. 2015 · Cell culture. Human monocytic THP-1 cells were maintained in culture in Roswell Park Memorial Institute medium (RPMI 1640, Invitrogen) culture medium containing 10 % of heat inactivated fetal bovine serum (Invitrogen) and supplemented with 10 mM Hepes (Gibco, #15630-056), 1 mM pyruvate (Gibco, #11360-039), 2.5 g/l D-glucose … " - Human monocytic cell line

Human monocytic cell line

In Vitro Differentiation of Macrophages and Dendritic Cells

Web15 mrt. 1988 · A monocytic cell line, termed Mono Mac, was established from peripheral blood of a patient with monoblastic leukemia. Two clones, designated Mono Mac I and … Web24 mei 2024 · The human monocytic cell line THP-1 , the human myelogenous leukaemia cell line K562 and HEK293 hTLR4A-MD2-CD14 (Invivogen, San Diego, CA) were …

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WebAbstract. THP-1 is a human leukemia monocytic cell line, which has been extensively used to study monocyte/macrophage functions, mechanisms, signaling pathways, and nutrient … WebMaterial Safety Data Sheet or SDS for THP-1 NF-kappaB-eGFP Human Acute Monocytic Leukemia Cell Line SCC223 from Merck for download or viewing in the browser. …

WebMany quinazoline derivatives with pharmacological properties, such as anticancer activity, have been synthesized. Fourteen quinazoline derivatives bearing a substituted sulfonamide moiety (4a–n) were previously synthesized and fully characterized. These compounds exerted antiproliferative activity against cell lines derived from solid tumors. … WebArticle Snippet: Human acute monocytic leukemia cell line THP-1 (ATCC TIB-202) was cultured in RPMI 1640 medium (HyClone, Logan, UT, USA) with 10% fetal bovine serum (FBS; Gibco, Carlsbad, CA, USA) at 37 °C in 5% CO 2 . Techniques: Infection, Expressing. Journal: International Journal of Biological Sciences.

WebMONO-MAC-6 - Cell line: MONO-MAC-6 DSMZ no.: ACC 124Species validation: human (Homo sapiens)Cell type: acute monocytic leukemia Origin: established from the … WebAbstract. THP-1 is a human leukemia monocytic cell line, which has been extensively used to study monocyte/macrophage functions, mechanisms, signaling pathways, and …

Web5 jun. 2015 · In this study, we utilized the monocytic cell line THP-1 as an in vitro latency model for human cytomegalovirus infection (HCMV). Characterization of THP-1 cells by immunofluorescence andWestern blot analysis confirmed the expression of all major ND10 components. THP-1 cells with a stable, individual knockdown of PML, hDaxx or Sp100 …

WebArticle Snippet: Murine macrophage cell J774A.1, RAW264.1, and human monocytic cell lines THP1 and U937 were procured from American Type Culture Collection and … charlene abraham npWeb1 okt. 2000 · THP-1 is a human monocytic cell line, 9 which matures into macrophage-like adherent cells following stimulation with phorbol 12-myristate 13-acetate 10 or 1α, 25-dihydroxy vitamin D3. 11 Cirillo et al.12 reported that L. pneumophila could invade and multiply within THP-1 cells in a manner similar to that in human monocytes and … harry patterson authorWebA human leukemic cell line (THP-1) cultured from the blood of a boy with acute monocytic leukemia is described. This cell line had Fc and C3b receptors, but no … harry patterson obituaryWebTHP-1 monocytes Engineered Human Reporter Monocytes THP1 reporter cells are derived from THP-1, a human monocytic cell line that naturally expresses many pattern-recognition receptors, including Toll-like receptors. InvivoGen offers several engineered THP-1 cell lines: charlene abeytaWeb27 apr. 2010 · It was subsequently shown using human epithelial and monocytic cell lines [86,87,88] that Shiga toxin-mediated modification of the ribosome triggered not only the activation of the JNK pathway, but the activation of the p38 mitogen-activated protein kinase (p38 MAPK) and extracellular-signaling regulated kinase (ERK) pathways. charlene a carruthersWebArticle Snippet: The human monocytic leukemia cell line THP-1 (ATCC, Manassas, USA) was expanded in 75 cm 2 culture flasks (Greiner Bio-One, Kremsmünster, Austria) in RPMI 1640 (Gibco, Carlsbad, USA) supplemented with 10% heat-inactivated fetal calf serum (FCS) (Gibco) and 1% penicillin-streptomycin (Gibco) in an incubator (37°C, 5% CO 2 , … charlene abrahamsWebMonocytic Leukemia Cell Line Infection of a monocytic leukemia cell line in vitro, however, induces a Th2-type response in both the cell lines and in peripheral blood mononuclear cells ex vivo. From: Zika and Other Neglected and Emerging Flaviviruses, 2024 View all Topics Add to Mendeley Download as PDF About this page charlene abrams