WebNov 9, 2024 · WAT (∼500 mg) and liver (50 mg) were homogenized in cell extraction buffer (Thermo Fisher Scientific; FNN0011) supplemented with 200 mM PMSF (Fisher BP231), 200 mM NaF (Sigma S6776), 200 mM sodium orthovanadate (Sigma S6508), and protease inhibitor cocktail, according to the manufacturer’s instructions (Sigma P-2714) at a ratio … Web• G418, 50mg/ml (Thermo Scientific, Fisher Scientific cat.# SC30069) • Dimethylsulfoxide (DMSO) (Fisher Scientific, cat.# BP231) • Dulbecco´s Phosphate-Buffered Saline (PBS), w/o calcium, magnesium, or Phenol Red (Thermo Scientific, Fisher . Scientific cat.# SH30028) • Angiotensin I human acetate hydrate (Sigma-Aldrich, cat.# A9650)
S1P Redistribution Assay
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WebGlycine – Fisher Scientific – Catalog # G46 DMSO – Fisher Scientific – Catalog # BP231 Heparin – Alfa Aesar – Catalog # A16198 Normal Donkey Serum – Jackson Immunoresearch – Catalog #: 017-000-121 *Tween is a registered trademark of Croda International PLC. WebDMSO Fisher BP231 Crystal violet Sigma HT90132 Ethanol PHARMCO 111000200 6 well plates Corning 3506 Tissue culture flasks Thermo Scientific 168509/156367 HUVEC media Sciencell 1001 . Author: Bright,Scott James Created Date: 7/21/2024 11:13:32 AM ... Web• Trypsin-EDTA, 0.05% (Thermo Scientific, Fisher Scientific cat.# SH30236) • G418, 50mg/ml (Thermo Scientific, Fisher Scientific cat.# SC30069) • Dimethylsulfoxide (DMSO) (Fisher Scientific, cat.# BP231) • Dulbecco´s Phosphate-Buffered Saline (PBS), w/o calcium, magnesium, or Phenol Red (Thermo Scientific, Fisher . Scientific cat ... how do you spell intimacy